Shameik MooreMiles Morales (voice)
Hailee SteinfeldGwen Stacy (voice)
Brian Tyree HenryJeff Morales (voice)
Luna Lauren VelezRio Morales (voice)
Jake JohnsonPeter B. Parker (voice)
Oscar IsaacMiguel O'Hara (voice)
Jason SchwartzmanSpot (voice)
Issa RaeJessica Drew (voice)
Daniel KaluuyaHobie Brown (voice)
Karan SoniPavitr Prabhakar (voice)
Shea WhighamGeorge Stacy (voice)
Greta LeeLYLA (voice)
Mahershala AliUncle Aaron (voice)
Amandla StenbergMargo Kess: Spider-Byte (voice)
Jharrel JeromeMiles G. Morales (voice)
Andy SambergBen Reilly (voice)
Jack QuaidPeter Parker (voice)
Rachel DratchMs. Weber (voice)
Ziggy MarleyLenny (voice)
Jorma TacconeAdriano Tumino / '67 (voice)
J.K. SimmonsJ. Jonah Jameson (voice)
Donald GloverAaron Davis
Elizabeth PerkinsMay / Quippy Spider-Person (voice)
Kathryn HahnDoc Ock (voice)
Ayo EdebiriGlory (voice)
Nicole DelaneyMJ (voice)
Antonina LentiniBetty (voice)
Atsuko OkatsukaYuri (voice)
Peter SohnGanke (voice)
Melissa SturmMary Jane (voice)
Lorraine VelezMaria (voice)
Nic NovickiLego Spider-Man (voice)
Taran KillamWeb-Slinger (voice)
Metro BoominMetro Spider-Man (voice)
Josh KeatonSpectacular Spider-Man (voice)
Sofia BarclayMalala Windsor: Spider-UK (voice)
Danielle PerezCharlotte Webber: Sun-Spider (voice)
Yuri LowenthalInsomniac Spider-Man (voice)
Rita Rani AhujaAdditional Voices (voice)
Ismail BasheyAdditional Voices (voice)
Oscar CamachoAdditional Voices (voice)
Freddy FerrariAdditional Voices (voice)
Kerry GutierrezAdditional Voices (voice)
Kamal KhanAdditional Voices (voice)
Angelo Sekou KouyateAdditional Voices (voice)
Andrew LevitonAdditional Voices (voice)
David MichieAdditional Voices (voice)
Sumit NaigAdditional Voices (voice)
Juan PachecoAdditional Voices (voice)
Chrystee PharrisAdditional Voices (voice)
Ben PronskyAdditional Voices (voice)
Al RodrigoAdditional Voices (voice)
Stan SellersAdditional Voices (voice)
Warren SrokaAdditional Voices (voice)
Jasper Johannes AndrewsAdditional Voices (voice)
Gredel Berrios CalladineAdditional Voices (voice)
Natalia CastellanosAdditional Voices (voice)
Russell Tyre FrancisAdditional Voices (voice)
Deepti GuptaAdditional Voices (voice)
Sohm KapilaAdditional Voices (voice)
Pradnya KuwadekarAdditional Voices (voice)
Ashley LondonAdditional Voices (voice)
Christopher MillerAdditional Voices (voice)
Andrea NavedoAdditional Voices (voice)
Lakshmi PatelAdditional Voices (voice)
Jacqueline PinolAdditional Voices (voice)
Eliyas QureshiAdditional Voices (voice)
Lashana RodriguezAdditional Voices (voice)
Jaswant Dev ShresthaAdditional Voices (voice)
Libby Thomas DickeyAdditional Voices (voice)
Jason Linere-WhiteAdditional Voices (voice)
Sitara AttaieAdditional Voices (voice)
Mayuri BhandariAdditional Voices (voice)
June ChristopherAdditional Voices (voice)
Michelle Jubilee GonzalezAdditional Voices (voice)
Marabina JaimesAdditional Voices (voice)
Rez KemptonAdditional Voices (voice)
Lex LangAdditional Voices (voice)
Phil LordAdditional Voices (voice)
Richard MiroAdditional Voices (voice)
Doug NicholasAdditional Voices (voice)
Shakira Ja'nai PayeAdditional Voices (voice)
James PirriAdditional Voices (voice)
Marley RalphAdditional Voices (voice)
Michelle RuffAdditional Voices (voice)
Dennis SingletaryAdditional Voices (voice)
Amanda TroopAdditional Voices (voice)
Ruth ZalduondoAdditional Voices (voice)
Kimberly BaileyAdditional Voices (voice)
Sanjay ChandaniAdditional Voices (voice)
Melanie DukeAdditional Voices (voice)
Jorge R. GutierrezAdditional Voices (voice)
Miguel JironAdditional Voices (voice)
Deepti Kingra-MickelsenAdditional Voices (voice)
Luisa LeschinAdditional Voices (voice)
Caitlin McKennaAdditional Voices (voice)
Andrew MorgadoAdditional Voices (voice)
Arthur OrtizAdditional Voices (voice)
Eliana A. PerezAdditional Voices (voice)
Juan PopeAdditional Voices (voice)
Mike RiandaAdditional Voices (voice)
Erika ScopelliAdditional Voices (voice)
Narender SoodAdditional Voices (voice)
Cedric L. WilliamsAdditional Voices (voice)
Kimiko GlennPeni Parker (voice) (uncredited)
Peggy LuMrs. Chen (uncredited)